Double‐Immunodiffusion Assay for Detecting Specific Antibodies

  title={Double‐Immunodiffusion Assay for Detecting Specific Antibodies},
  author={Peter V. Hornbeck},
  journal={Current Protocols in Immunology},
  • P. Hornbeck
  • Published 1 December 1991
  • Biology
  • Current Protocols in Immunology
Double immunodiffusion owes its success to the unique nature of antibody‐antigen interactions. When polyvalent antibodies with moderate‐to‐high intrinsic affinities are mixed with antigen at the right ratio (called the zone of equivalence) lattices of antibody‐antigen complexes form and precipitate out of solution. When, as described in this unit, gradients of antigen and antibody are established by diffusion from adjacent wells in a bed of agar, a line of practically insoluble precipitation… 

Isotype Determination of Antibodies

Several methods of class or isotype determination are outlined in this unit: sandwich ELISA, electrophoresis, and immunofixation, or use of a variety of commercially available kits.

Isotype Determination of Antibodies

This unit describes two methods for measurement and classification of supernatant or serum immunoglobulins: an ELISA and a method employing electrophoresis and immunofixation.

Production of Polyclonal Antisera

The production of polyclonal antisera specific for protein antigens in rabbits, rats, mice, and hamsters is described and a method for preparing serum from blood is presented.

Antigen-Antibody Interaction

The purpose of this experiment is to illustrate how antigen-antibody interactions can be applied to the study of evolution and to support the current classification of the ungulates at the order and suborder level.

An in-house indirect immunofluorescence test for ensuring that the cell bank is contamination free

Purity of three batches of MRC5 working seed cell was verified using in- house IIF test and no contamination to continuous cell lines was found and 100% intra-laboratory reproducibility showed.

Development of monospecific polyclonal antibodies against hypervirulent Klebsiella pneumoniae

Purified polyclonal antiseras against K. pneumoniae serotypes K1 and K2 showed high affinity and specificity for homologous antigens in immunodiffusion and direct agglutination tests, and immunohistochemical staining.

Antinuclear Antibodies in Systemic Sclerosis: an Update

Systemic sclerosis is an autoimmune disease characterized by fibrosis of skin and internal organs, vasculopathy, and dysregulation of immune system. A diagnostically important feature of

Characterization of a Novel Influenza A Virus Hemagglutinin Subtype (H16) Obtained from Black-Headed Gulls

A previously unidentified antigenic subtype of HA (H16), detected in viruses circulating in black-headed gulls in Sweden, is described and proposed that sequence analyses of HA and NA genes of influenza A viruses be used for the rapid identification of existing and novel HA andNA subtypes.

Novel polyol‐responsive monoclonal antibodies against extracellular β‐d‐glucans from Pleurotus ostreatus

β‐d‐glucans from mushroom strains play a major role as biological response modifiers in several clinical disorders. Therefore, a specific assay method is of critical importance to find useful and

Neutralization by "antineoplastin" of insulin-activated nitric oxide synthase antibody and its effects in cancers

It was found that the resumption of NO synthesis through the neutralization of antibody resulted in favorable modifications of various cancer-associated pathophysiologic consequences.



Immuno - diffusion and immunoelectrophoresis

  • Hand - book of Experimental Immunology
  • 1986