Double‐Immunodiffusion Assay for Detecting Specific Antibodies

@article{Hornbeck2001DoubleImmunodiffusionAF,
  title={Double‐Immunodiffusion Assay for Detecting Specific Antibodies},
  author={Peter V. Hornbeck},
  journal={Current Protocols in Immunology},
  year={2001},
  volume={00}
}
  • P. Hornbeck
  • Published 1 December 1991
  • Biology
  • Current Protocols in Immunology
Double immunodiffusion owes its success to the unique nature of antibody‐antigen interactions. When polyvalent antibodies with moderate‐to‐high intrinsic affinities are mixed with antigen at the right ratio (called the zone of equivalence) lattices of antibody‐antigen complexes form and precipitate out of solution. When, as described in this unit, gradients of antigen and antibody are established by diffusion from adjacent wells in a bed of agar, a line of practically insoluble precipitation… 

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