Dot-enzyme-linked immunosorbent assay (Dot-ELISA) of anti-Entamoeba histolytica antibodies in human serum and colostrum.

Abstract

Seroepidemiologic and clinical studies of amoebiasis require improved methods for the detection and titration of anti-Entamoeba histolytica (Eh) trophozoite antibodies. We have developed a Dot-enzyme-linked immunosorbent assay (Dot-ELISA) to titrate anti-Eh antibodies in human serum and colostrum. The anti-Eh serum IgG antibody titers from healthy individuals and from patients with amoebic liver abscess obtained, with our Dot-ELISA were similar to those obtained with a standard ELISA, but the background values were lower. Significant anti-Eh sIgA antibody titers were found in seven out of 42 colostrum samples of mothers of newborn children with diarrhea. Our Dot-ELISA is rapid, inexpensive, and might be used for the diagnosis of individuals and in seroepidemiologic studies of amoebiasis.

Cite this paper

@article{Revilla1991DotenzymelinkedIA, title={Dot-enzyme-linked immunosorbent assay (Dot-ELISA) of anti-Entamoeba histolytica antibodies in human serum and colostrum.}, author={R L{\'o}pez Revilla and Fernando Navarro-Garc{\'i}a and Margarita Valadez-S{\'a}nchez and Yolanda L{\'o}pez Vidal and J Calva Mercado}, journal={Archivos de investigación médica}, year={1991}, volume={22 3-4}, pages={249-53} }