Dose response and proliferative characteristics of aberrant crypt foci: putative preneoplastic lesions in rat colon.

@article{McLellan1991DoseRA,
  title={Dose response and proliferative characteristics of aberrant crypt foci: putative preneoplastic lesions in rat colon.},
  author={E A McLellan and Alan Medline and Ranjana P. Bird},
  journal={Carcinogenesis},
  year={1991},
  volume={12 11},
  pages={
          2093-8
        }
}
Foci of aberrant crypts (ACF) have been identified in the unsectioned methylene blue stained rodent colons and hypothesized to represent precursor lesions of colon cancer. In the present study, induction and growth characteristics of ACF were investigated in response to a single injection of varying dosages of 1,2-dimethylhydrazine-2HCl (DMH), a colon carcinogen. Female Sprague-Dawley rats were given a single injection of DMH (5-150 mg/kg). Two and 19 weeks after the injection, animals were… 

Characterisation of aberrant crypt foci in carcinogen-treated rats: association with intestinal carcinogenesis.

Mulin production in the unsectioned colon of rats was studied, demonstrating that ACFs producing sulphomucins were progressively reduced when ACF multiplicity increased, whereas ACFs containing sialomucin-producing ACs increased with crypt multiplicity.

Azoxymethane-Induced Aberrant Crypt Foci and Colorectal Tumors in F344 Rats: Sequential Analysis of Growth

In this study, ACF growing features and distribution are not correlated to adenoma and adenocarcinoma distribution, and it is conceivable that signet ring cell carcinomas arising in the proximal colon, where ACF are rare, could present a different pathway of growth.

Ability of aberrant crypt foci characteristics to predict colonic tumor incidence in rats fed cholic acid.

The main finding of this study is that the number of ACF at early time points did not predict tumor incidence and crypt multiplicity was a consistent predictor of tumor outcome and should be measured in future studies using ACF as a biological end point.

Colonic Tumor Incidence in Rats Fed Cholic Acid Ability of Aberrant Crypt Foci Characteristics to Predict Updated

The main finding of this study is that the number of ACF at early time points did not predict tumor incidence and crypt multiplicity was a consistent predictor of tumor outcome and should be measured in future studies using ACF as a biological end point.

Increased Cell Proliferation of Azoxymethane‐induced Aberrant Crypt Foci of Rat Colon

The presence of many cells stained by PCNA in the upper portion of ACF suggested that ACF have more G1 phase cells, which readily respond to mitogenic stimulation, than G0 phase cells which are predominant in normal crypts.

Aberrant crypt foci and colon tumors in F344 rats have similar increases in proliferative activity

The mean proliferative activity of aberrant crypt foci in the distal colons was found to be 3‐to 4‐fold over that of the adjacent normal crypts at every time period analyzed and was comparable to that seen in benign and malignant colon tumors in the same animals.

Azoxymethane-induced rat aberrant crypt foci: relevance in studying chemoprevention of colon cancer.

  • Jayadev Raju
  • Biology, Medicine
    World journal of gastroenterology
  • 2008
The scope of this mini-review is to provide valuable insights and limitations of AOM-induced rat ACF as biomarker in colon cancer chemoprevention studies, and the role of the dynamics and biological heterogeneity of ACF is critical in understanding them as biomarkers in chemoplevention studies.

Aberrant crypt foci.

The advances in ACF research as precursors of CRCs are highlighted and various proteomic (Prot) markers may be altered within ACF suggesting possible prospective pathological changes.
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