Dose-dependent induction of recessive mutations with N-ethyl-N-nitrosourea in primordial germ cells of male mice.

@article{Shibuya1996DosedependentIO,
  title={Dose-dependent induction of recessive mutations with N-ethyl-N-nitrosourea in primordial germ cells of male mice.},
  author={T Tsunefumi Shibuya and N Horiya and Hideyasu Matsuda and Kiyoshi Sakamoto and Takumi Hara},
  journal={Mutation research},
  year={1996},
  volume={357 1-2},
  pages={219-24}
}
Using a specific locus test, we previously found that N-ethyl-N-nitrosourea (ENU) induces recessive mutations at a relatively high rate in male mouse primordial germ cells (PGC) at 8.5, 10.5 and 13.5 days of development (G8.5, G10.5 and G13.5). A large difference was observed on the induced mutation rate between 30 and 50 mg/kg ENU in 10.5-day PGC. We therefore carried out specific locus tests to ascertain whether ENU induces recessive mutations in a dose-dependent manner in G8.5 and G10.5 PGC… CONTINUE READING