Dorsal-Mediated Repression Requires the Formation of a Multiprotein Repression Complex at the Ventral Silencer

@article{Valentine1998DorsalMediatedRR,
  title={Dorsal-Mediated Repression Requires the Formation of a Multiprotein Repression Complex at the Ventral Silencer},
  author={Scott A. Valentine and Guoqing Chen and Tatiana Shandala and Joseph Fernandez and Sheenah M. Mische and Robert Saint and Albert J Courey},
  journal={Molecular and Cellular Biology},
  year={1998},
  volume={18},
  pages={6584 - 6594}
}
ABSTRACT Dorsal functions as both an activator and repressor of transcription to determine dorsoventral fate in the Drosophila melanogaster embryo. Repression by Dorsal requires the corepressor Groucho (Gro) and is mediated by silencers termed ventral repression regions (VRRs). A VRR in zerknüllt(zen) contains Dorsal binding sites as well as an essential element termed AT2. We have identified and purified an AT2 DNA binding activity in embryos and shown it to consist of cut(ct) and dead ringer… Expand
Capicua is involved in Dorsal-mediated repression of zerknüllt expression in Drosophila embryo
TLDR
Evidence is presented that Capicua (Cic) is involved in Dl-mediated repression in the zerknüllt (zen) ventral repression element (VRE) and that Dl failed to repress zen expression in lateral regions of cic mutant embryos, suggesting that Cic may function as a corepressor by binding the VRE. Expand
Activation and repression by the C-terminal domain of Dorsal.
TLDR
It is found that activation by Dorsal, in particular by the C-terminal domain, is modulated by the maternal terminal pattern-forming system. Expand
Uncoupling Dorsal-mediated activation from Dorsal-mediated repression in the Drosophila embryo
TLDR
Although the dedicated activator alleles fail to repress decapentaplegic and zerknüllt in the syncytial blastoderm embryo, they are able to pattern the dorsoventral axis, indicating that dor soventral patterning is not dependent upon Dorsal-mediated repression, reflecting the existence of redundant mechanisms to block DecapentapLegic signaling. Expand
Receptor tyrosine kinase signaling regulates different modes of Groucho-dependent control of Dorsal
TLDR
The interplay between DI, Gro and Dri on the hkb enhancer is examined and it is shown that when acting over a distance, Gro abolishes rather than converts DI activator function. Expand
Mechanisms of Groucho-mediated repression revealed by genome-wide analysis of Groucho binding and activity
BackgroundThe transcriptional corepressor Groucho (Gro) is required for the function of many developmentally regulated DNA binding repressors, thus helping to define the gene expression profile ofExpand
Transcriptional repression: the long and the short of it.
TLDR
Examples of both long-range and short-range repression are discussed, showing that long- range repression may often involve the assembly of a multiprotein complex termed a repressosome that is analogous in many ways to the enhanceosomes known to mediate activation. Expand
A functional interaction between the histone deacetylase Rpd3 and the corepressor groucho in Drosophila development.
TLDR
These findings support the view that Gro mediates repression, at least in part, by the direct recruitment of the histone deacetylase Rpd3 to the template, where it can modulate local chromatin structure. Expand
Relief of gene repression by torso RTK signaling: role of capicua in Drosophila terminal and dorsoventral patterning.
TLDR
Evidence that Tor signaling regulates terminal patterning by inactivating Cic at the embryo poles is presented, suggesting that Cic-like proteins may act as repressors regulated by RTK signaling in other organisms. Expand
A Role for Groucho Tetramerization in Transcriptional Repression
  • Guoqing Chen, Pierre H. Nguyen, A. Courey
  • Medicine, Biology
  • Molecular and Cellular Biology
  • 1998
TLDR
It is determined, via a variety of hydrodynamic measurements as well as protein cross-linking, that native Gro is a tetramer in solution and that tetramerization is mediated by two putative amphipathic α-helices (termed leucine zipper-like motifs) found in the N-terminal region. Expand
Groucho/TLE family proteins and transcriptional repression.
The Drosophila Groucho (Gro) protein is the prototype for a large family of corepressors, examples of which are found in most metazoans. This family includes the human transducin-like Enhancer ofExpand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 49 REFERENCES
Conversion of a silencer into an enhancer: evidence for a co‐repressor in dorsal‐mediated repression in Drosophila.
TLDR
It is found that T‐rich sequences close to the dl binding sites in the silencer region of the zen promoter are conserved between three Drosophila species, indicating that the minimal silencer was converted into an enhancer. Expand
Binding sites for transcription factor NTF-1/Elf-1 contribute to the ventral repression of decapentaplegic.
TLDR
A corepression element in decapentaplegic (dpp), a zygotically active gene that is repressed by the Dorsal morphogen is identified, and one of the DREs in the dpp VRR overlaps the binding site for a potential activator protein suggesting that one mechanism of ventral repression may be the mutually exclusive binding of repressor and activator proteins. Expand
Conversion of a dorsal‐dependent silencer into an enhancer: evidence for dorsal corepressors.
TLDR
Results suggest that dl is intrinsically an activator, but is converted into a potent silencer when it interacts with neighboring corepressors. Expand
The same dorsal binding site mediates both activation and repression in a context‐dependent manner.
TLDR
It is found that when multiple copies of a dorsal binding site from the zerknüllt ventral repressor element are fused to a heterologous basal promoter, the resulting construct is activated by dorsal to give a ventral specific expression pattern. Expand
Conversion of dorsal from an activator to a repressor by the global corepressor Groucho.
TLDR
It is shown that recruitment of Groucho to the template by protein:protein interactions is required for the conversion of Dorsal from an activator to a repressor and is therefore a critical component of the dorsal/ventral patterning system. Expand
Individual dorsal morphogen binding sites mediate activation and repression in the Drosophila embryo.
TLDR
This is the first demonstration that common binding sites for any DNA binding protein can mediate both activation and repression in a developing embryo and it is presented that the affinities of dl binding sites are important for the efficiency of repression, but are not the sole determinants of the threshold response to the dl gradient. Expand
The interplay between multiple enhancer and silencer elements defines the pattern of decapentaplegic expression.
TLDR
It is demonstrated here that multiple regions within the second intron of the gene cooperate with one another to generate the wild-type level and pattern of dpp transcription, indicating that the same boundary of repression can be specified by dl-binding sites of different affinity. Expand
The dorsal morphogen is a sequence-specific DNA-binding protein that interacts with a long-range repression element in drosophila
TLDR
It is shown that dl is a DNA-binding protein that specifically interacts with distal sequences of the zerknüllt (zen) promoter, one of the genetic targets of the morphogen. Expand
Control of tailless expression by bicoid, dorsal and synergistically interacting terminal system regulatory elements
Three different maternal morphogen gradients regulate expression of the gap gene tailless (tll), which is required to establish the acron and telson of the Drosophila embryo. To identify elements inExpand
Functional analysis of the Drosophila twist promoter reveals a dorsal-binding ventral activator region.
TLDR
Using P-element-mediated germ-line transformation, a small dorsal-dependent ventral activator region (VAR) is mapped in the 5'-flanking region of the twist promoter that can direct the early ventral expression of a heterologous promoter. Expand
...
1
2
3
4
5
...