Dopamine receptors in equine ovarian tissues.

  title={Dopamine receptors in equine ovarian tissues.},
  author={Sheryl S King and Andrew G Campbell and Elizabeth A Dille and Janet F. Roser and Laura L. Murphy and Kristen L Jones},
  journal={Domestic animal endocrinology},
  volume={28 4},
Dopamine (DA) agonist and antagonist treatments can affect ovarian reproductive events in the mare. To support our theory that DA produces these effects by acting directly on the ovary, we analyzed equine ovarian tissues for the presence of dopamine receptor-1 (D1r) and dopamine receptor-2 (D2r) mRNA by reverse transcription polymerase chain reaction (RT-PCR) and D1r and D2r proteins by Western blot and immunohistochemistry (IHC). RT-PCR was performed on RNA isolated from ovarian cortex… CONTINUE READING