Dopamine is released spontaneously from developing midbrain neurons in organotypic culture

@article{Cragg1998DopamineIR,
  title={Dopamine is released spontaneously from developing midbrain neurons in organotypic culture},
  author={Stephanie J. Cragg and C. J. Holmes and Christopher Hawkey and Susan A. Greenfield},
  journal={Neuroscience},
  year={1998},
  volume={84},
  pages={325-330}
}
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12 Citations
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12 Citations

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Midbrain dopaminergic neurons generate calcium and sodium currents and release dopamine in the striatum of pups
TLDR
It is shown that half of identified E16–P0 mDA neurons spontaneously generate non-synaptic, intrinsically driven Ca2+ spikes andCa2+ plateaus mediated by N- and L-type voltage-gated Ca2 + channels, which suggests that mouse striatal dopaminergic synapses are functional at birth.
Release of endogenous dopamine in cultured mesencephalic neurons: influence of dopaminergic agonists and glucocorticoid antagonists
TLDR
Coulometry is applied to the study of dopamine release in primary cultures of embryonic mesencephalic dopaminergic neurons to demonstrate an in vitro model that allows the studying of the release of endogenous dopamine in cell cultures and the effects of glucocorticoid hormones on the release dynamics.
Anatomical and functional reconstruction of the nigrostriatal system in vitro: Selective innervation of the striatum by dopaminergic neurons
TLDR
Results show that cocultures of cortex–striatum and mesencephalic slices result in a system that displays a number of the morphological and functional traits of the normal nigrostriatal system and that can be relied on as a good in vitro model of in vivo development.
Synaptic release of dopamine in the subthalamic nucleus
TLDR
It is suggested that SN DA neurons directly modulate the activity of subthalamic nucleus neurons and their loss may contribute to the abnormal activity of STN neurons in PD.
Dopaminergic neurons develop axonal projections to their target areas in organotypic co-cultures of the ventral mesencephalon and the striatum/prefrontal cortex
The reverse transport of DA, what physiological significance?
  • V. Leviel
  • Biology
    Neurochemistry International
  • 2001
Dopamine release in organotypic cultures of foetal mouse mesencephalon: effects of depolarizing agents, pargyline, nomifensine, tetrodotoxin and calcium
TLDR
Ex vivo endogenous DA release was assessed by high‐performance liquid chromatography in organotypic cultures of foetal mouse (E12) midbrain following single or multiple challenges with high K+ or veratridine in the presence or absence of pargyline, nomifensine, calcium and/or tetrodotoxin.
Synaptic basis of persistent activity in prefrontal cortex in vivo and in organotypic cultures.
TLDR
The stereotyped nature of the multiple-component persistent activity pattern reported here might be a common feature of interconnected cortical networks but within PFC could be related to the persistent activity required for working memory.
Action potential and calcium dependence of tonic somatodendritic dopamine release in the Substantia Nigra pars compacta
TLDR
The findings indicate that although tonic somatodendritic DA release is largely independent of action potentials, basal [DA]out is strongly regulated by voltage‐dependent Ca2+ influx and release of intracellular Ca2+.
Neuronal degeneration and reorganization: a mutual principle in pathological and in healthy interactions of limbic and prefrontal circuits.
TLDR
A view of plasticity that promotes a link between hippocampal and prefrontal structure and function is proposed, and serious damage can be internalized without simultaneous disruption of neural dynamics offering an approach to reverse dysfunctional reorganization in the prefrontal cortex.
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References

SHOWING 1-10 OF 25 REFERENCES
Characteristics of electrically evoked somatodendritic dopamine release in substantia nigra and ventral tegmental area in vitro.
TLDR
The findings that somatodendritic release was frequency dependent across a range characteristic of DA cell firing rates and that stimulated [DA]o varied markedly among DA cell body regions have important implications for how dendritically released DA may function in the physiology and pathophysiology of substantia nigra and VTA.
The influence of target and non-target brain regions on the development of mid-brain dopaminergic neurons in organotypic slice culture.
Evidence for the absence of impulse-regulating somatodendritic and synthesis-modulating nerve terminal autoreceptors on subpopulations of mesocortical dopamine neurons
D1-type dopamine receptors inhibit growth cone motility in cultured retina neurons: evidence that neurotransmitters act as morphogenic growth regulators in the developing central nervous system.
TLDR
The experiments provide strong support for the hypothesis that neurotransmitters, besides mediating transynaptic signaling in the adult, may have a role in neuronal differentiation as growth regulators.
Development of Transmitter‐Releasing Capacity in Neuron‐Enriched Tissue Cultures
TLDR
Results show that neuron‐enriched cultures respond to depolarizing stimuli in a manner similar to excised adult basal ganglia tissue, with the appearance of functional ionic channels after 3 days in vitro.
Dopamine and serotonin inhibition of neurite elongation of different identified neurons
TLDR
It is demonstrated that a second classical neurotransmitter, dopamine, can act to suppress regenerative neurite outgrowth and the implications of neurotransmitter regulation of the dynamics of neuronal morphology are discussed.
Differential Autoreceptor Control of Somatodendritic and Axon Terminal Dopamine Release in Substantia Nigra, Ventral Tegmental Area, and Striatum
TLDR
Data indicate that autoinhibition via D2receptors consistently plays a less significant role in the control of somatodendritic than axon terminal DA release, and that even at the level of som atodendrites themselves, D2 autoin inhibition displays marked regional variation.
Regulation of transient dopamine concentration gradients in the microenvironment surrounding nerve terminals in the rat striatum
Heterogeneity of electrically evoked dopamine release and reuptake in substantia nigra, ventral tegmental area, and striatum.
TLDR
The heterogeneous patterns of DA uptake within SNc and VTA were consistent with the pattern of degeneration in Parkinson's disease; less vulnerable DA cells, e.g., those in VTA, have less DA uptake than the more vulnerable cells of caudal SNc, and Uptake was less efficient in SNcand VTA than in striatum.
Electrophysiological and pharmacological evidence for the existence of distinct subpopulations of nigrostriatal dopaminergic neuron in the rat
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