Dopamine is released spontaneously from developing midbrain neurons in organotypic culture

@article{Cragg1998DopamineIR,
  title={Dopamine is released spontaneously from developing midbrain neurons in organotypic culture},
  author={Stephanie J. Cragg and C. J. Holmes and Christopher Hawkey and Susan A. Greenfield},
  journal={Neuroscience},
  year={1998},
  volume={84},
  pages={325-330}
}
Midbrain dopaminergic neurons generate calcium and sodium currents and release dopamine in the striatum of pups
TLDR
It is shown that half of identified E16–P0 mDA neurons spontaneously generate non-synaptic, intrinsically driven Ca2+ spikes andCa2+ plateaus mediated by N- and L-type voltage-gated Ca2 + channels, which suggests that mouse striatal dopaminergic synapses are functional at birth.
Release of endogenous dopamine in cultured mesencephalic neurons: influence of dopaminergic agonists and glucocorticoid antagonists
TLDR
Coulometry is applied to the study of dopamine release in primary cultures of embryonic mesencephalic dopaminergic neurons to demonstrate an in vitro model that allows the studying of the release of endogenous dopamine in cell cultures and the effects of glucocorticoid hormones on the release dynamics.
Anatomical and functional reconstruction of the nigrostriatal system in vitro: Selective innervation of the striatum by dopaminergic neurons
TLDR
Results show that cocultures of cortex–striatum and mesencephalic slices result in a system that displays a number of the morphological and functional traits of the normal nigrostriatal system and that can be relied on as a good in vitro model of in vivo development.
Synaptic release of dopamine in the subthalamic nucleus
TLDR
It is suggested that SN DA neurons directly modulate the activity of subthalamic nucleus neurons and their loss may contribute to the abnormal activity of STN neurons in PD.
Dopamine release in organotypic cultures of foetal mouse mesencephalon: effects of depolarizing agents, pargyline, nomifensine, tetrodotoxin and calcium
TLDR
Ex vivo endogenous DA release was assessed by high‐performance liquid chromatography in organotypic cultures of foetal mouse (E12) midbrain following single or multiple challenges with high K+ or veratridine in the presence or absence of pargyline, nomifensine, calcium and/or tetrodotoxin.
Synaptic basis of persistent activity in prefrontal cortex in vivo and in organotypic cultures.
TLDR
The stereotyped nature of the multiple-component persistent activity pattern reported here might be a common feature of interconnected cortical networks but within PFC could be related to the persistent activity required for working memory.
Action potential and calcium dependence of tonic somatodendritic dopamine release in the Substantia Nigra pars compacta
TLDR
The findings indicate that although tonic somatodendritic DA release is largely independent of action potentials, basal [DA]out is strongly regulated by voltage‐dependent Ca2+ influx and release of intracellular Ca2+.
Neuronal degeneration and reorganization: a mutual principle in pathological and in healthy interactions of limbic and prefrontal circuits.
TLDR
A view of plasticity that promotes a link between hippocampal and prefrontal structure and function is proposed, and serious damage can be internalized without simultaneous disruption of neural dynamics offering an approach to reverse dysfunctional reorganization in the prefrontal cortex.
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