Don't put all your eggs in one basket: a cost-effective and powerful method to optimize primer choice for rRNA environmental community analyses using the Fluidigm Access Array.

Abstract

With the increasing democratization of high-throughput sequencing (HTS) technologies, along with the concomitant increase in sequence yield per dollar, many researchers are exploring HTS for microbial community ecology. Many elements of experimental design can drastically affect the final observed community structure, notably the choice of primers for… (More)
DOI: 10.1111/1755-0998.12507

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@article{Brown2016DontPA, title={Don't put all your eggs in one basket: a cost-effective and powerful method to optimize primer choice for rRNA environmental community analyses using the Fluidigm Access Array.}, author={Shawn P. Brown and Astrid Ferrer and James W. Dalling and Katy D Heath}, journal={Molecular ecology resources}, year={2016}, volume={16 4}, pages={946-56} }