Dominant-negative p53 mutations in rheumatoid arthritis.

  title={Dominant-negative p53 mutations in rheumatoid arthritis.},
  author={Zuoning Han and David L. Boyle and Yuan Shi and Douglas R Green and Gary S. Firestein},
  journal={Arthritis and rheumatism},
  volume={42 6},
OBJECTIVE Studies were performed to determine if p53 mutations identified in rheumatoid arthritis (RA) synovial tissue are dominant negative. METHODS Site-directed mutagenesis was used to produce 2 RA-derived mutants: asparagine-->serine at codon 239 (N239S) and arginine-->stop at codon 213 R213*). HS68 dermal fibroblasts were transfected with either empty vector, wild-type p53 cDNA (wt), or the N239S or R213* mutant p53 cDNA clones. Interleukin-6 (IL-6) and bax gene expression were… CONTINUE READING


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