Dominant-negative C/EBP disrupts mitotic clonal expansion and differentiation of 3T3-L1 preadipocytes.

@article{Zhang2004DominantnegativeCD,
  title={Dominant-negative C/EBP disrupts mitotic clonal expansion and differentiation of 3T3-L1 preadipocytes.},
  author={Jiang-Wen Zhang and Qiqun Tang and Charles Vinson and Melissa D. Lane},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2004},
  volume={101 1},
  pages={43-7}
}
Hormonal induction of growth-arrested 3T3-L1 preadipocytes rapidly activates expression of CCAAT/enhancer-binding protein (C/EBP) beta. Acquisition of DNA-binding activity by C/EBPbeta, however, is delayed until the cells synchronously enter the S phase of mitotic clonal expansion (MCE). After MCE, C/EBPbeta activates expression of C/EBPalpha and peroxisome proliferator-activated receptor gamma, which then transcriptionally activate genes that give rise to the adipocyte phenotype. A-C/EBP… CONTINUE READING