Dominant inhibitory mutations in the Mg(2+)-binding site of RasH prevent its activation by GTP.

@article{Farnsworth1991DominantIM,
  title={Dominant inhibitory mutations in the Mg(2+)-binding site of RasH prevent its activation by GTP.},
  author={Charles L. Farnsworth and Larry A. Feig},
  journal={Molecular and cellular biology},
  year={1991},
  volume={11 10},
  pages={
          4822-9
        }
}
We have previously demonstrated that substitution of Asn for Ser at position 17 of RasH yields a dominant inhibitory protein whose expression in cells interferes with endogenous Ras function (L. A. Feig, and G. M. Cooper, Mol. Cell. Biol. 8:3235-3243, 1988). Subsequent structural studies have shown that the hydroxyl group of Ser-17 contributes to the binding of Mg2+ associated with bound nucleotide. In this report, we show that more subtle amino acid substitutions at this site that would be… CONTINUE READING
BETA

Citations

Publications citing this paper.
SHOWING 1-10 OF 48 CITATIONS

Similar Papers

Loading similar papers…