Domains in cell plasma membranes investigated by near-field scanning optical microscopy.

  title={Domains in cell plasma membranes investigated by near-field scanning optical microscopy.},
  author={J M Hwang and Levi A Gheber and Leonid Margolis and Michael A. Edidin},
  journal={Biophysical journal},
  volume={74 5},
Near-field scanning optical microscopy (NSOM) uses the near-field interaction of light from a sharp fiber-optic probe with a sample of interest to image surfaces at a resolution beyond the diffraction limit of conventional optics. We used NSOM to image fluorescently labeled plasma membranes of fixed human skin fibroblasts, either dried or in buffer. A patchy distribution of a fluorescent lipid analog suggestive of lipid domains was observed in the fixed, dried cells. The sizes of these patches… CONTINUE READING


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