Domain shuffling as a tool for investigation of protein function: substitution of the cysteine-rich region of Raf kinase and PKC eta for that of yeast Pkc1p.

@article{Schmitz2001DomainSA,
  title={Domain shuffling as a tool for investigation of protein function: substitution of the cysteine-rich region of Raf kinase and PKC eta for that of yeast Pkc1p.},
  author={H P Schmitz and Johannes J{\"o}ckel and Christoph Block and J{\"u}rgen J. Heinisch},
  journal={Journal of molecular biology},
  year={2001},
  volume={311 1},
  pages={1-7}
}
With the completion of the sequences of entire genomes, the need for functional characterisation of proteins and their domains is becoming acute. Conserved regions within proteins often share overlapping functions but despite this conservation may fulfil quite different tasks in different species. In this work, we investigated the cysteine-rich motif (C1 domain) of yeast protein kinase C (Pkc1p) as a model to establish a test system for domain function. C1 domains activate kinases through… CONTINUE READING
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