Domain-directed polymerase chain reaction capable of distinguishing bacterial from host DNA at the single-cell level: characterization of a systematic method to investigate putative bacterial infection in idiopathic disease.

@article{Steinman1997DomaindirectedPC,
  title={Domain-directed polymerase chain reaction capable of distinguishing bacterial from host DNA at the single-cell level: characterization of a systematic method to investigate putative bacterial infection in idiopathic disease.},
  author={Charles R. Steinman and B Muralidhar and Gerard J Nuovo and Peter Rumore and De-min Yu and Masahiro Mukai},
  journal={Analytical biochemistry},
  year={1997},
  volume={244 2},
  pages={328-39}
}
The use of a broad-range PCR to target conserved sequences in the bacterial ribosome has long been recognized as an approach to investigating idiopathic human diseases for the presence of bacteria. An important example is rheumatoid arthritis where the hypothesis of a bacterial etiology remains viable despite failure of previous methods to identify a causative organism. Practical implementation of this strategy, however, was impeded by requirements unique to the study of these diseases. Hence… CONTINUE READING