Domain conservation in several volvocalean cell wall proteins

  title={Domain conservation in several volvocalean cell wall proteins},
  author={Jeffrey P. Woessner and Arthur J. Molendijk and Piet van Egmond and Frans Klis and Ursula W. Goodenough and Michel A Haring},
  journal={Plant Molecular Biology},
Based on our previous work demonstrating that (SerPro)x epitopes are common to extensin-like cell wall proteins in Chlamydomonas reinhardtii, we looked for similar proteins in the distantly related species C. eugametos. Using a polyclonal antiserum against a (SerPro)10 oligopeptide, we found distinct sets of stage-specific polypeptides immunoprecipitated from in vitro translations of C. eugametos RNA. Screening of a C. eugametos cDNA expression library with the antiserum led to the isolation of… CONTINUE READING