Domain analysis of human U5 RNA. Cap trimethylation, protein binding, and spliceosome assembly.

@article{Hinz1996DomainAO,
  title={Domain analysis of human U5 RNA. Cap trimethylation, protein binding, and spliceosome assembly.},
  author={Matthias Hinz and Melissa J. Moore and Albrecht Bindereif},
  journal={The Journal of biological chemistry},
  year={1996},
  volume={271 31},
  pages={19001-7}
}
We have analyzed the sequence requirements of the human U5 RNA during small nuclear ribonucleoprotein (snRNP) and spliceosome assembly. A collection of mutant derivatives of the human U5 RNA gene was constructed in a U1 expression vector and transiently transfected in mammalian cells. Using immunoprecipitation and affinity selection assays, the cap… CONTINUE READING