Dolichol‐linked oligosaccharide and glycoprotein biosyntheses in glial cells in primary culture: Development and enzymatic correlates

@article{Ishii1988DolichollinkedOA,
  title={Dolichol‐linked oligosaccharide and glycoprotein biosyntheses in glial cells in primary culture: Development and enzymatic correlates},
  author={S Ishii and Joseph J. Volpe},
  journal={Journal of Neuroscience Research},
  year={1988},
  volume={20}
}
  • S. Ishii, J. Volpe
  • Published 1 August 1988
  • Biology, Medicine
  • Journal of Neuroscience Research
Primary cultures of cerebral glia derived from neonatal rat brain were utilized to determine (1) the developmental changes of dolichol‐linked oligosaccharide and N‐linked glycoprotein biosyntheses, (2) the enzymatic correlates of these developmental changes, and (3) the temporal relations between the biosyntheses of dolichol‐oligosaccharide and N‐linked glycoproteins and the biochemical expression of astrocytic and oligodendroglial differentiation. Marked, parallel developmental increases in… Expand
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References

SHOWING 1-10 OF 50 REFERENCES
Dolichol-linked glycoprotein synthesis in developing mammalian brain: maturational changes of the N-acetylglucosaminylphosphotransferase.
TLDR
The data suggest a temporally discrete period of activation of the dolichol-linked pathway to glycoprotein biosynthesis, whether the pathway is regulated coordinately or sequentially is a fertile topic for future study. Expand
Dolichol‐Linked Glycoprotein Synthesis in G1 Is Necessary for DNA Synthesis in Synchronized Primary Cultures of Cerebral Glia
TLDR
There is a requirement for dolichol‐linked glycoprotein synthesis for the subsequent occurrence of DNA synthesis and that this requirement is expressed late in the G1 phase of the cell cycle. Expand
Enzymatic Regulation of Glycoprotein Synthesis in Peripheral Nervous System Myelin
TLDR
Results indicate an important role for the enzyme UDP‐N‐acetylglucosamine:dolichyl phosphate, N‐acetelglucOSamine‐1‐phosphate transferase in the synthesis of P0 in peripheral nerve myelin and that the content of the lipid cofactor may limit glycoprotein synthesis in vivo. Expand
Postnatal changes in dolichol-pathway enzyme activities in cerebral cortex neurons.
TLDR
There is an increase in the glycosylation of asparagine-type glycoproteins during synapse formation in the neurons of the cerebral cortex. Expand
Glial differentiation in dissociated cell cultures of neonatal rat brain: Noncoordinate and density‐dependent regulation of oligodendroglial enzymes
TLDR
The investigation was addressed specifically to the possibility of noncoordinate regulation of two manifestations of oligodendroglial differentiation, i.e., activities of glycerol‐3‐phosphate dehydrogenase and 2′,3′‐cyclic nucleotide 3-phosphohydrolase, as well as the effects of initial cell density on the time of onset and the intensity of expression. Expand
A developmental change in dolichyl phosphate mannose synthase activity in pig brain.
TLDR
These studies strongly suggest that the developmental change in dolichyl phosphate mannose synthase activity is due primarily to the presence of a relatively lower amount of endogenous dlichyl monophosphate being accessible to the mannosyltransferase in the white-matter membranes from adult animals. Expand
CARBOHYDRATE‐PEPTIDE LINKAGES IN GLYCOPROTEINS AND MUCOPOLYSACCHARIDES FROM BRAIN
TLDR
The results indicate that N‐acetylgalactosamine at the reducing end of oligosaccharide chains in brain glycoproteins is linked O‐glycosidically to the hydroxyl groups of serine and threonine residues. Expand
Cholesterol Biosynthesis and Its Regulation in Dissociated Cell Cultures of Fetal Rat Brain: Developmental Changes and the Role of 3‐Hydroxy‐3‐Methylglutaryl Coenzyme A Reductase
TLDR
Primary cultures of cells dissociated from fetal rat brain were utilized to define the developmental changes in cholesterol biosynthesis and the role of HMG‐CoA reductase in the regulation of these changes, suggesting an intimate relationship of the sterol biosynthetic pathway with cellular proliferation and with oligodendroglial differentiation in developing mammalian brain. Expand
Dolichol kinase and the regulation of dolichyl phosphate levels in developing brain.
TLDR
Dolichol kinase is the principal determinant of cellular levels of dolichyl phosphate, the critical intermediate in the dolICHol-linked pathway to glycoproteins. Expand
Noncoordinate regulation of myelinogenic parameters in primary cultures of dissociated fetal rat brain
TLDR
There are multiple parallel but separate mechanisms of temporal regulation controlling myelinogenic gene expression, and that, among them, those factors that control MBP accumulation are more sensitive to disruption by the rigors of dissociated culture. Expand
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