Does the synthesis of ribosomal RNA take place within nucleolar fibrillar centers or dense fibrillar components? A critical appraisal.

@article{Raska1995DoesTS,
  title={Does the synthesis of ribosomal RNA take place within nucleolar fibrillar centers or dense fibrillar components? A critical appraisal.},
  author={Ivan Raska and M Dundr and Karel Koberna and Ivo Mel{\vc}{\'a}k and Mar{\'i}a Del Carmen Risue{\~n}o and Istv{\'a}n T{\"o}r{\"o}k},
  journal={Journal of structural biology},
  year={1995},
  volume={114 1},
  pages={1-22}
}
The localization of transcribing rRNA genes within nucleoli of mammalian cells, although intensively studied, has not been established. Most published papers on this topic situate transcribing ribosomal genes either to nucleolar fibrillar centers or to nucleolar dense fibrillar components. To clarify this point, we have generated the electron microscopic affinity cytochemistry picture of the nucleolus of cultured mammalian cells. Three kinds of affinity probes have been used: (1) probes to… CONTINUE READING