Docetaxel load biodegradable porous microspheres for the treatment of colorectal peritoneal carcinomatosis.

Abstract

Micro- and nanoparticle formulations are widely used to improve the bioavailability of low solubility drugs. In this study, biodegradable poly(L-lactide acid)-Pluronic L121-poly(L-lactide acid) (PLLA-L121-PLLA) was developed. And then a controlled drug delivery system (CDDS), docetaxel (DOC) loaded PLLA-L121-PLLA porous microsphere (DOC MS) was prepared for colorectal peritoneal carcinomatosis (CRPC) therapy. DOC MS was prepared by DOC and PLLA-L121-PLLA using an oil-in-water emulsion solvent evaporation method. The particle size, morphological characteristics, encapsulation efficiency, in vitro drug release studies and in vitro cytotoxicity of DOC MS have been investigated. In vitro release profile demonstrated a significant difference between rapid release of free DOC and much slower and sustained release of DOC MS. Furthermore, cytotoxicity assay indicated cytotoxicity was increased after DOC was encapsulated into polymeric microspheres. In addition, intraperitoneal administration of DOC MS could effectively suppress growth and metastasis of CT26 peritoneal carcinomatosis in vivo, and prolonged the survival of tumor bearing mice. Immunohistochemistry staining of tumor tissues with Ki-67 revealed that DOC MS induced a stronger anti-tumor effect by increasing apoptosis of tumor cells in contrast to other groups (P<0.05). Thus, our results suggested that DOC MS may have great potential applications in clinic.

DOI: 10.1016/j.ijbiomac.2014.05.026

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Cite this paper

@article{Fan2014DocetaxelLB, title={Docetaxel load biodegradable porous microspheres for the treatment of colorectal peritoneal carcinomatosis.}, author={Rangrang Fan and Yuelong Wang and Bo Han and Youfu Luo and Liangxue Zhou and Xirui Peng and Min Wu and Yu Zheng and Gang Guo}, journal={International journal of biological macromolecules}, year={2014}, volume={69}, pages={100-7} }