Do processing time and storage of sputum influence quantitative bacteriology in bronchiectasis?

@article{Murray2010DoPT,
  title={Do processing time and storage of sputum influence quantitative bacteriology in bronchiectasis?},
  author={Maeve P Murray and Catherine J. Doherty and John R. W. Govan and Adam T. Hill},
  journal={Journal of medical microbiology},
  year={2010},
  volume={59 Pt 7},
  pages={829-33}
}
This study aimed to establish whether the bacterial density of spontaneous sputum is affected by the time and mode of sample storage. Ten patients with bronchiectasis collected all sputum expectorated over 45 min. The samples were aliquoted and processed at 25 degrees C for qualitative and quantitative bacteriology at 1, 2, 4 and 6 h from expectoration. Further aliquots were stored at 25 degrees C, 4 degrees C and -20 degrees C for 24 and 48 h prior to processing. The species present was… CONTINUE READING