Do fluorescence decays remitted from tissues accurately reflect intrinsic fluorophore lifetimes?

Abstract

Fluorescence spectroscopy and imaging methods, including fluorescence lifetime sensing, are being developed for noninvasive tissue diagnostics. The purpose of this study was to identify and quantify those factors affecting the accurate recovery of fluorophore lifetimes from inhomogeneous tissues in vivo. A Monte Carlo code was developed to numerically… (More)

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