DnrD cyclase involved in the biosynthesis of doxorubicin: purification and characterization of the recombinant enzyme.

@article{Kendrew1999DnrDCI,
  title={DnrD cyclase involved in the biosynthesis of doxorubicin: purification and characterization of the recombinant enzyme.},
  author={Steven G. Kendrew and Kinya Katayama and Edward Deutsch and Krishnamurthy Madduri and C. Richard Hutchinson},
  journal={Biochemistry},
  year={1999},
  volume={38 15},
  pages={
          4794-9
        }
}
Mutations in the Streptomyces peucetius dnrD gene block the ring cyclization leading from aklanonic acid methyl ester (AAME) to aklaviketone (AK), an intermediate in the biosynthetic pathway to daunorubicin (DNR) and doxorubicin. To investigate the role of DnrD in this transformation, its gene was overexpressed in Escherichia coli and the DnrD protein was purified to homogeneity and characterized. The enzyme was shown to catalyze the conversion of AAME to AK presumably via an intramolecular… CONTINUE READING