DnaK heat shock protein of Escherichia coli maintains the negative supercoiling of DNA against thermal stress.

@article{Ogata1996DnaKHS,
  title={DnaK heat shock protein of Escherichia coli maintains the negative supercoiling of DNA against thermal stress.},
  author={Yasuyuki Ogata and Tsunekazu Mizushima and Kazuhiro Kataoka and Kiyoshi Kita and Takeyoshi Miki and Kazuhisa Sekimizu},
  journal={The Journal of biological chemistry},
  year={1996},
  volume={271 46},
  pages={29407-14}
}
Plasmid DNA in exponentially growing Escherichia coli immediately relaxes after heat shock, and the relaxed state of DNA rapidly reverts to the original state with exposure to conditions of heat shock. We have now obtained genetic and biochemical evidence indicating that DnaK heat shock protein of E. coli, a prokaryotic homologue of hsp70, is involved in this re-supercoiling of DNA. As re-supercoiling of DNA did not occur in an rpoH amber mutant, it seems likely that heat shock proteins are… CONTINUE READING

From This Paper

Topics from this paper.
10 Citations
0 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 10 extracted citations

Similar Papers

Loading similar papers…