DnaK as a thermometer: threonine-199 is site of autophosphorylation and is critical for ATPase activity.

@article{McCarty1991DnaKAA,
  title={DnaK as a thermometer: threonine-199 is site of autophosphorylation and is critical for ATPase activity.},
  author={John S McCarty and Graham C Walker},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1991},
  volume={88 21},
  pages={9513-7}
}
DnaK, the sole Escherichia coli member of the highly conserved 70-kDa heat shock protein (HSP70) family of proteins, autophosphorylates when incubated with ATP in vitro. We show that threonine-199 is the amino acid that becomes phosphorylated and we demonstrate that threonine-199 is critical for the ATPase activity of DnaK. We also report that both the ATPase and autophosphorylating activities of DnaK increase very strongly over the range of temperatures that is physiologically relevant for E… CONTINUE READING