DnaA-stimulated transcriptional activation of ori l : Escherichia coli RNA polymerase b subunit as a transcriptional activator contact site

@inproceedings{SzalewskaPaasz1998DnaAstimulatedTA,
  title={DnaA-stimulated transcriptional activation of ori l : Escherichia coli RNA polymerase b subunit as a transcriptional activator contact site},
  author={Agnieszka Szalewska-Pałasz and Alicja Węgrzyn and A Błaszczak and Karol Taylor and Grzegorz Węgrzyn},
  year={1998}
}
We present evidence that Escherichia coli RNA polymerase b subunit may be a transcriptional activator contact site. Stimulation of the activity of the pR promoter by DnaA protein is necessary for replication of plasmids derived from bacteriophage l. We found that DnaA activates the pR promoter in vitro. Particular mutations in the rpoB gene were able to suppress negative effects that certain dnaA mutations had on the replication of l plasmids; this suppression was allele-specific. When a… CONTINUE READING
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