Dna stability and survival of bacillus subtilis spores in extreme dryness

  title={Dna stability and survival of bacillus subtilis spores in extreme dryness},
  author={Klaus Dose and Markus Gill},
  journal={Origins of Life and Evolution of the Biosphere},
  • K. Dose, M. Gill
  • Published 1 June 1995
  • Biology
  • Origins of Life and Evolution of the Biosphere
The inactivation of Bacillus subtilis spores during long-term exposure (up to several months) to extreme dryness (especially vacuum) is strain-dependent, through only to a small degree. During a first phase (lasting about four days) monolayers of spores lose about 20% of their viability, regardless of the strain studied. During this phase loss in viability can be equally attributed both to damages of hydrophobic structures (membranes and proteins) and DNA. During a second phase lasting for the… 

Response ofBacillus subtilis spores to dehydration and UV irradiation at extremely low temperatures

The data indicate that the low temperatures that prevail in the outer parts of the Solar System or at the nightside of Mars or the Moon are not sufficiently low to crucially inhibit inactivation by dehydration, and place further constraints on the panspermia hypothesis.

Germination and inactivation of Bacillus subtilis spores induced by moderate hydrostatic pressure

High pressure was necessary and very efficient in inducing spore germination, however, it seemed to slow the enzymatic digestion of the cortex, which is required for germinated spores to be inactivated by pressure.

Single-cell analysis reveals individual spore responses to simulated space vacuum

Examining spores’ molecular changes under simulated space vacuum using micro-Raman spectroscopy found that this vacuum did not cause significant denaturation of spore protein, and new insight is given into individual spore’s responses to space vacuum and new techniques for microorganism analysis at the single-cell level are provided.

Survival of Microorganisms under the Extreme Conditions of the Atacama Desert

The solar fluences causing 63% loss in viability (F37-values) have been determined and data are discussed with respect to possible similarities between the climatic conditions of the recent Atacama Desert and the deserts of early Mars.

[Resistance to Dehydratation of Extremophilic Bacteria from Antarctic Region and Hypersaline Reservoirs].

The results support the hypothesis that between bacteria resistance to electromagnetic radiation (UV) and their resistance to dehydratation there is a correlation.

Novel methods for storage stability and release of Bacillus spores

Formulations of Bacillus spores with acacia gum and porous carriers (charcoal and tapioca) prolong the anticipated shelf‐life of spores even under ambient temperature and provide slow and steady bacterial release consistent with their high viability.

Adaptation of Bacillus subtilis cells to Archean-like UV climate: relevant hints of microbial evolution to remarkably increased radiation resistance.

In a precursory study for the space experiment ADAPT ("Molecular adaptation strategies of microorganisms to different space and planetary UV climate conditions"), cells of Bacillus subtilis 168 were

Survival of the Halophilic Archaeon Halovarius luteus after Desiccation, Simulated Martian UV Radiation and Vacuum in Comparison to Bacillus atrophaeus

A novel haloarchaea isolated from Urmia Salt Lake, Iran, Halovarius luteus strain DA50T, was exposed to varying levels of simulated extraterrestrial conditions and compared to that of the bacteria Bacillus atrophaeus, which showed the greatest decrease in viability.



Survival in extreme dryness and DNA-single-strand breaks.

I will survive: protecting and repairing spore DNA.

In order for spores to survive during these long periods of dormancy, mechanisms must be operating to prevent accumulation of potentially lethal damage in spore DNA.

DNA-strand breaks limit survival in extreme dryness

The inactivation of the anhdrobiotic organismsBacillus subtilis (spores) and Deinococcus radiodurans during long-term exposure to extreme dryness is correlated with an increase in the number of DNA-strand breaks and other DNA lesions.

Extreme dryness and DNA-protein cross-links.

Localization of low-molecular-weight basic proteins in Bacillus megaterium spores by cross-linking with ultraviolet light

Significant amounts of the low-molecular-weight basic proteins unique to bacterial spores are associated with spore DNA in vivo, according to dose-response curves for formation of covalent cross-links.

Responses ofBacillus subtilis spores to space environment: Results from experiments in space

  • G. Horneck
  • Physics
    Origins of life and evolution of the biosphere
  • 2005
Onboard of several spacecrafts, spores of Bacillus subtilis were exposed to selected parameters of space, such as space vacuum, different spectral ranges of solar UV-radiation and cosmic rays, applied separately or in combination, and their survival and genetic changes after retrieval are studied.

Anhydrobiosis: a strategy for survival.

  • L. CroweJ. Crowe
  • Biology
    Advances in space research : the official journal of the Committee on Space Research
  • 1992