Division and gametophytic tissue formation from protoplasts of young sporophytes in fern Lygodium japonicum

Abstract

Protoplasts isolated enzymatically from juvenile leaflets of sporophytes in fern Lyqodium japonicum were inoculated in a MS medium containing NAA 2.7 μm, BAP 2.2 μm, 0.6 M mannitol, and 0.05 M sucrose. Cell division took place within 8 days of culture initiation, and after 30 days cell-clusters with 10–15 cells were observed. When the cell-clusters were… (More)
DOI: 10.1007/BF00232083

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