Divinylsulphone-activated agarose. Formation of stable and non-leaking affinity matrices by immobilization of immunoglobulins and other proteins.

Abstract

Divinylsulphone-activated agarose is an attractive alternative to several of the activated supports usually used. Unlike CNBr-activated gels, it does not leak the immobilized protein at high pH. It reacts readily with proteins at near-neutral pH (unlike the epoxy-activated supports). Generally, divinylsulphone-activated agarose reacts with amino, hydroxyl, and sulphydryl groups, thus allowing immobilization of a wide spectrum of ligands. Moreover, it is available in an aqueous suspension free of organic solvents and neither requires time-consuming swelling nor washing.

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@article{Lihme1986DivinylsulphoneactivatedAF, title={Divinylsulphone-activated agarose. Formation of stable and non-leaking affinity matrices by immobilization of immunoglobulins and other proteins.}, author={Allan Lihme and Claus Schafer-Nielsen and Kiss Larsen and Kathryn G Mueller and Thorkild C. B\og-Hansen}, journal={Journal of chromatography}, year={1986}, volume={376}, pages={299-305} }