Diversity of Grapevine fanleaf virus isolates from Iran.

  title={Diversity of Grapevine fanleaf virus isolates from Iran.},
  author={Nemat Sokhandan Bashir and Shaheen Nourinejhad Zarghani and Mohammad saeid Hejazi},
  journal={Virus research},
  volume={128 1-2},
Enzyme-linked immunosorbent assay (ELISA) testing of 126 grapevine samples, from vineyards in the northwest region of Iran, detected Grapevine fanleaf virus (GFLV) in 33 samples. Total RNA from eight of the infected samples were subjected to reverse transcription polymerase chain reaction (RT-PCR) analysis using primers which corresponded to the virus coat protein and 3' non coding region of RNA 2. An expected 1620 bp DNA fragment was amplified from all the tested samples. PCR products from… CONTINUE READING