Diversity in the rates of transcript elongation by single RNA polymerase molecules.

@article{ToliNrrelykke2004DiversityIT,
  title={Diversity in the rates of transcript elongation by single RNA polymerase molecules.},
  author={Simon F Toli{\'c}-N\orrelykke and Anita M. Engh and Robert Landick and Jeff Gelles},
  journal={The Journal of biological chemistry},
  year={2004},
  volume={279 5},
  pages={3292-9}
}
Single-molecule measurements of the activities of a variety of enzymes show that rates of catalysis may vary markedly between different molecules in putatively homogeneous enzyme preparations. We measured the rate at which purified Escherichia coli RNA polymerase moves along a approximately 2650-bp DNA during transcript elongation in vitro at 0.5 mm nucleoside triphosphates. Individual molecules of a specifically biotinated RNA polymerase derivative were tagged with 199-nm diameter avidin… CONTINUE READING
39 Extracted Citations
0 Extracted References
Similar Papers

Citing Papers

Publications influenced by this paper.
Showing 1-10 of 39 extracted citations

Similar Papers

Loading similar papers…