Diverse microglial motility behaviors during clearance of dead cells in hippocampal slices.

@article{Petersen2004DiverseMM,
  title={Diverse microglial motility behaviors during clearance of dead cells in hippocampal slices.},
  author={Mark A. Petersen and Michael E. Dailey},
  journal={Glia},
  year={2004},
  volume={46 2},
  pages={195-206}
}
We used two-channel three-dimensional time-lapse fluorescence confocal imaging in live rat hippocampal slice cultures (1-7 days in vitro) to determine the motility behaviors of activated microglia as they engage dead and dying cells following traumatic brain tissue injury. Live microglia were labeled with a fluorescently conjugated lectin (IB(4)), and dead neurons were labeled with a membrane-impermeant fluorescent DNA-binding dye (Sytox Orange or To-Pro-3). Tissue injury during the slicing… CONTINUE READING

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