Diverse endonucleolytic cleavage sites in the mammalian transcriptome depend upon microRNAs, Drosha, and additional nucleases.

@article{Karginov2010DiverseEC,
  title={Diverse endonucleolytic cleavage sites in the mammalian transcriptome depend upon microRNAs, Drosha, and additional nucleases.},
  author={Fedor V. Karginov and Sihem Cheloufi and Mark M. W. Chong and Alexander Stark and Andrew D Smith and Gregory J Hannon},
  journal={Molecular cell},
  year={2010},
  volume={38 6},
  pages={781-8}
}
The life span of a mammalian mRNA is determined, in part, by the binding of regulatory proteins and small RNA-guided complexes. The conserved endonuclease activity of Argonaute2 requires extensive complementarity between a small RNA and its target and is not used by animal microRNAs, which pair with their targets imperfectly. Here we investigate the endonucleolytic function of Ago2 and other nucleases by transcriptome-wide profiling of mRNA cleavage products retaining 5' phosphate groups in… CONTINUE READING