Divergent transcription of pdxB and homology between the pdxB and serA gene products in Escherichia coli K-12.

@article{Schoenlein1989DivergentTO,
  title={Divergent transcription of pdxB and homology between the pdxB and serA gene products in Escherichia coli K-12.},
  author={Patricia Veronica Schoenlein and Benjamin B. Roa and Malcolm E Winkler},
  journal={Journal of bacteriology},
  year={1989},
  volume={171 11},
  pages={6084-92}
}
We report the DNA sequence and in vivo transcription start of pdxB, which encodes a protein required for de novo biosynthesis of pyridoxine (vitamin B6). The DNA sequence confirms results from previous minicell experiments showing that pdxB encodes a 41-kilodalton polypeptide. RNase T2 mapping of in vivo transcripts and corroborating experiments with promoter expression vector pKK232-8 demonstrated that the pdxB promoter shares its -10 region with an overlapping, divergent promoter. Thus, pdxB… CONTINUE READING
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