Divergence in urinary 8-iso-PGF(2alpha) (iPF(2alpha)-III, 15-F(2t)-IsoP) levels from gas chromatography-tandem mass spectrometry quantification after thin-layer chromatography and immunoaffinity column chromatography reveals heterogeneity of 8-iso-PGF(2alpha). Possible methodological, mechanistic an

@article{Tsikas2003DivergenceIU,
  title={Divergence in urinary 8-iso-PGF(2alpha) (iPF(2alpha)-III, 15-F(2t)-IsoP) levels from gas chromatography-tandem mass spectrometry quantification after thin-layer chromatography and immunoaffinity column chromatography reveals heterogeneity of 8-iso-PGF(2alpha). Possible methodological, mechanistic an},
  author={Dimitrios Tsikas and Edzard Schwedhelm and Maria Theresia Suchy and Jonas Niemann and Frank Mathias Gutzki and Veit Johannes Erpenbeck and J M Hohlfeld and Andrzej Surdacki and J{\"u}rgen C. Fr{\"o}lich},
  journal={Journal of chromatography. B, Analytical technologies in the biomedical and life sciences},
  year={2003},
  volume={794 2},
  pages={237-55}
}
Free radical-catalysed oxidation of arachidonic acid esterified to lipids leads to the formation of the F(2)-isoprostane family which may theoretically comprise up to 64 isomers. We have previously shown that the combination of TLC and GC-tandem MS (referred to as method A) allows for the accurate and highly specific quantification of 8-iso-PGF(2alpha) (iPF(2alpha)-III, 15-F(2t)-IsoP) in human urine. Immunoaffinity column chromatography (IAC) with immobilized antibodies raised against 8-iso-PGF… CONTINUE READING

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