Dithiothreitol enhances Listeria monocytogenes mediated cell cytotoxicity.

  title={Dithiothreitol enhances Listeria monocytogenes mediated cell cytotoxicity.},
  author={David G. Westbrook and Arun K. Bhunia},
  journal={Microbiology and immunology},
  volume={44 6},
The hybridoma Ped-2E9 based cytotoxicity assay was developed to distinguish virulent from avirulent Listeria species in 6 hr. The cytotoxicity effect on Ped-2E9 was reported to be primarily due the cytolytic action of listeriolysin O (LLO), produced by L. monocytogenes. In this study, the effect of a reducing agent, dithiothreitol (DTT, 0-2 mM) that is known to activate LLO was investigated to make the Ped-2E9 based cytotoxicity assay an even more sensitive and rapid. Also, we examined the… CONTINUE READING