Disulfide bond reduction corresponds to dimerization and hydrophobicity changes of Clostridium botulinum type A neurotoxin

AbstractAim:To determine the structure factors that mediate the intoxication process of botulinum neurotoxin type A (BoNT/A).Methods:Triton X-114 phase separation experiments and 1-anilino-8-naphthalene sulfonate binding assay were used to study the structural factor that corresponds to the hydrophobicity change of BoNT/A. In addition, sucrose density… CONTINUE READING