Distribution pattern of GPRC6A mRNA in mouse tissue by in situ hybridization.

@article{Luo2010DistributionPO,
  title={Distribution pattern of GPRC6A mRNA in mouse tissue by in situ hybridization.},
  author={Jun-ming Luo and Zhaoqian Liu and Jinshi Liu and Chin Y Eugene},
  journal={Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences},
  year={2010},
  volume={35 1},
  pages={1-10}
}
OBJECTIVE To explore the distribution pattern of G protein-coupled receptor family C, group 6, subtype A (GPRC6A) mRNA in adult mice. METHODS The distribution of GPRC6A mRNA in paraffin embedded adult mouse tissues was determined by highly sensitive nonradioactive cRNA probe in situ hybridization (ISH). We compared ISH with and without addition of tyramide signal amplification (TSA). GPRC6A wild-type and littermate GPRC6A null mice tissue sections were investigated by ISH. RESULTS TSA… CONTINUE READING