Distribution of wheat germ agglutinin in young wheat plants.

  title={Distribution of wheat germ agglutinin in young wheat plants.},
  author={Michael L. Mishkind and Kenneth Keegstra and Barry A. Palevitz},
  journal={Plant physiology},
  volume={66 5},
A liquid phase, competition-binding radioimmunoassay for wheat germ agglutinin, with a detection limit of 10 nanograms, was developed in order to determine the distribution of this lectin in young wheat plants. Affinity columns for wheat germ agglutinin removed all antigenically detectable activity from crude extracts of wheat tissue; thus, the antigenic cross-reactivity detected by the assay possesses sugar-binding specificity similar to the wheat germ-derived lectin. The amount of lectin per… CONTINUE READING
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The interaction of wheat agglutinin with sialoglycoproteins ; the role of sialic acid

  • J Biol Chem
  • 1979

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