• Corpus ID: 1420617

Distribution of the folate receptor GP38 in normal and malignant cell lines and tissues.

@article{Weitman1992DistributionOT,
  title={Distribution of the folate receptor GP38 in normal and malignant cell lines and tissues.},
  author={Steven D. Weitman and R H Lark and Leslie R. Coney and Daniel W. Fort and Verna Frasca and Vincent R. Zurawski and Barton A. Kamen},
  journal={Cancer research},
  year={1992},
  volume={52 12},
  pages={
          3396-401
        }
}
In some epithelial cells studied in vitro a membrane-bound folate receptor initiates the process for cell accumulation of 5-methyltetrahydrofolic acid. This receptor was found to be GP38, an overexpressed, glycosyl-phosphatidylinositol anchored glycoprotein, recognized by two monoclonal antibodies, designated MOv18 and MOv19. Using immunoblotting with MOv19, radioimmunoassay with MOv18 and 19, Northern blot analysis, and radioligand binding when possible, we describe the limited expression of… 

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...

References

SHOWING 1-10 OF 49 REFERENCES

Folate-binding protein is a marker for ovarian cancer.

The folate-binding protein locus was not amplified in any of the 16 carcinoma cell lines tested and in only 1 of 10 serous adenocarcinomas, indicating that overexpression of FBP in ovarian cancer cannot, in general, be due to gene amplification.

Complementary DNA for the folate binding protein correctly predicts anchoring to the membrane by glycosyl-phosphatidylinositol.

A cDNA library from a human carcinoma cell line, Caco-2, which expresses the membrane form abundantly was constructed and a near full-length cDNA for the folate binder was isolated, which deduced amino acid sequence is not consistent with a typical membrane spanning domain but rather with a signal for anchoring via a glycosyl-phosphatidylinositol linkage.

Isolation and characterization of a folate receptor from human placenta.

Immunofluorescent studies with this antiserum and human erythrocytes revealed the presence of an immunologically similar protein on the plasma membrane of these cells suggesting that this protein may function as a folate receptor.

Cloning of a tumor-associated antigen: MOv18 and MOv19 antibodies recognize a folate-binding protein.

Results indicate that the MOv18 and MOv19 monoclonal antibodies bind to at least one form of folate-binding protein and that this protein, which is evidently overexpressed in certain malignant tumors, may provide a suitable target for immunotherapy with these antibodies.

The Ca-MOv18 molecule, a cell-surface marker of human ovarian carcinomas, is anchored to the cell membrane by phosphatidylinositol.

Further observations on the folate-binding factor in some leukemic cells.

The lysates of peripheral cells as well as the serum from some patients with chronic myelogenous leukemia, contained a macromolecular factor which bound tritiated folic acid, suggesting that the binding of methotrexate may be a mechanism by which leukemic cells become resistant to this drug.

Receptor-mediated folate accumulation is regulated by the cellular folate content.

  • B. KamenA. Capdevila
  • Biology, Chemistry
    Proceedings of the National Academy of Sciences of the United States of America
  • 1986
Data indicate that cells possess a high-affinity, high-specificity folate receptor whose expression is regulated by the folate content of the cell, and suggest that a small molecule such as folate can enter cells by receptor-mediated endocytosis.

The folate receptor works in tandem with a probenecid-sensitive carrier in MA104 cells in vitro.

Using both probenecid and low temperature as selective inhibitors, this work has successfully blocked transmembrane movement of the vitamin into the cytoplasm without affecting binding to the receptor or the internalization of the Vitamin-receptor complex, which suggests that passage is through an anion carrier.

The folate-binding protein of rat kidney. Purification, properties, and cellular distribution.

Intense fluorescence staining for FBP was localized at the apices (brush border) of proximal tubules, and the choroid plexus, an organ previously shown to contain FBP, also exhibited intense fluorescent staining.