Distribution of the flavohaemoglobin, HMP, between periplasm and cytoplasm in Escherichia coli.

@article{Vasudevan1995DistributionOT,
  title={Distribution of the flavohaemoglobin, HMP, between periplasm and cytoplasm in Escherichia coli.},
  author={Subhash G. Vasudevan and Petrus Tang and Nicholas E Dixon and Robert K. Poole},
  journal={FEMS microbiology letters},
  year={1995},
  volume={125 2-3},
  pages={219-24}
}
The subcellular distribution of the soluble flavohaemoglobin (HMP) of Escherichia coli has been determined. Cells over-expressing HMP from the cloned hmp gene on a multicopy plasmid were fractionated by osmotic shock and lysozyme treatment. Spectral analysis of subcellular fractions showed the CO-binding haemoprotein to be cytoplasmic. However, Western blotting using antibody raised to purified HMP revealed approximately 30% of the protein to be periplasmic in the over-expressing strain… CONTINUE READING