Distribution of sialoglycoconjugates on the luminal surface of the endothelial cell in the fenestrated capillaries of the pancreas.

  title={Distribution of sialoglycoconjugates on the luminal surface of the endothelial cell in the fenestrated capillaries of the pancreas.},
  author={V. Mureşan and M. Constantinescu},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  pages={474 - 476}
  • V. Mureşan, M. Constantinescu
  • Published 1985
  • Biology, Medicine
  • The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
Sialic acid-bearing molecules on the luminal surface of the vascular endothelium in mouse and rat pancreatic capillaries were detected electron microscopically by using a procedure with ferritin hydrazide (FH), after preferential oxidation of sialyl residues with sodium periodate. The distribution of FH on the endothelial surface demonstrated the existence of microdomains with various densities of sialoglycoconjugates oxidizable by sodium periodate and accessible to the tracer. On the… Expand
Ultrastructural study of endothelial permeability to macromolecules in fetal paraplacental capillaries of European badger.
Permeability of the continuous placental capillaries to macromolecules has been studied in the hemophagous region of the badger placenta by perfusion in situ of cationized ferritin (CF) in threeExpand
High and low molecular weight tracers for the electron microscopical detection of sialoglycoconjugates
Results indicate that the three hydrazide-derivative tracers may be useful tools for the electron microscopical detection of cellular and extracellular sialoglycoconjugates. Expand
Receptor-Mediated Transcytosis of Plasma Molecules by Vascular Endothelium
Vascular endothelium is endowed with a wide spectrum of functions among which of paramount importance is the exchange of substances between blood and tissues. Endothelium possesses all the basicExpand
Appraisal of polyethyleneimine used as a tracer for anionic sites in human cardiac tissues with a brief reference to anionic molecular organization in altered basement membranes.
  • Y. S. Lee
  • Chemistry, Medicine
  • Journal of molecular and cellular cardiology
  • 1986
The anionic sites characterized by PEI which is considered to be superior to most polycationic colloids show a regular lattice-like arrangement in the basement membranes of human myocardial cells and capillaries. Expand
A hemagglutination test for binding of hydrazide-derivatized cytochemical markers.
A sensitive hemagglutination test was developed in order to predict the ability of amine- or hydrazide-derivatized cytochemical markers to interact with cell surface aldehydes in various in vitro andExpand
Wheatgerm agglutinin-mediated toxicity in pancreatic cancer cells
WGA proved highly toxic to human pancreatic carcinoma cells in vitro and the resulting toxicity was profound, and was profound in case of WGA (ID50 at 2.5–5 μg ml–1). Expand
Circulation of the pancreas and salivary glands
The sections in this article are: Anatomical Considerations, Functional Hyperemia, Circulatory Adjustments to Hyperplasia, and Pharmacology. Expand
Synergistic effects of cell-penetrating peptide Tat and fusogenic peptide HA2-enhanced cellular internalization and gene transduction of organosilica nanoparticles.
The in vitro and in vivo studies demonstrated that the synergistic effects of cell-penetrating peptide Tat and fusogenic peptide HA2 could promote the efficient cellular internalization, endosome escape, and nucleus targeting, hence delivering the therapeutic nucleic acid efficiently. Expand


Differentiated microdomains on the luminal surface of the capillary endothelium. II. Partial characterization of their anionic sites
The results indicate that the anionic sites of the fenestral diaphragms are contributed primarily by heparan sulfate and/or heparin, whereas those of the plasmalemma proper are of mixed chemical nature. Expand
Differentiated microdomains on the luminal surface of the capillary endothelium. I. Preferential distribution of anionic sites
Cationized ferritin binds preferentially to certain microdomains of the luminal plasmalemma of fenestrated capillaries (mouse pancreas and jejunum) and the functional implications of the association of these micro Domains with structures involved in capillary permeability are discussed. Expand
Distribution of sialoglycoconjugates on acinar cells of the mammalian pancreas.
Results suggest that LPA binding sites on the acinar cell apical surface may be primarily sialoglycoproteins, while those on the basolateral surfaces may consist in part of gangliosides. Expand
Differentiated microdomains on the luminal surface of capillary endothelium: distribution of lectin receptors
Lectins conjugated with either peroxidase or ferritin were used to detect specific monosaccharide residues on the luminal front of he fenestrated endothelium in the capillaries of murine pancreas and intestinal mucosa, complementing previous findings on the existence of microdomains generated by the preferential distribution of chemically different anionic sites. Expand
The Biological Role of Sialic Acid at the Surface of the Cell
Hirst (1945; 1948) was the first to suggest that the substrate for the “receptor destroying enzyme” of influenza virus, later recognized as sialidase (neuraminidase, see Chapter 10), was aExpand
Selective radioactive labeling of cell surface sialoglycoproteins by periodate-tritiated borohydride.
This simple method of specifically introducing a radioactive label into cell surface sialic acids is useful in the study of cell surfaceSialic acid-containing glycoproteins. Expand
Ferritin hydrazide, a novel covalent electron dense reagent for the ultrastructural localization of glycoconjugates.
This study represents the first example of covalent labeling of cell surfaces with an electron dense material and is much simpler then the three-step affinity cytochemical techniques based on avidin-biotin interaction. Expand
Purification and use of limulin: a sialic acid-specific lectin.
Hemagglutination inhibition studies with horse red blood cells indicate specificity of LPA for N-acetyl- and N-glycolylneuraminic acid; binding is Ca2+ -dependent and abolished by neuraminidase treatment. Expand
The sialoglycoproteins of murine erythrocyte ghosts. A modified periodic acid-Schiff stain procedure staining nonsubstituted and O-acetylated sialyl residues on glycopeptides.
Murine erythrocyte ghosts (from DBA/2, CD-1, and B6D2 strains) contain significant amounts of O-acetylated sialyl residues, which are slowly oxidized by periodate, and periodic acid-Schiff staining detects only one major sialoglycoprotein. Expand
Use offernitin hydrazide for the detection of sialoglycoconjugates . I . Methodological aspects
  • Rev Roum Biochim
  • 1984