Distribution of reaction products in phospholipase A2 hydrolysis.

@article{Wacklin2007DistributionOR,
  title={Distribution of reaction products in phospholipase A2 hydrolysis.},
  author={Hanna Pauliina Wacklin and Fredrik Tiberg and Giovanna Fragneto and Robert K Thomas},
  journal={Biochimica et biophysica acta},
  year={2007},
  volume={1768 5},
  pages={1036-49}
}
We have monitored the composition of supported phospholipid bilayers during phospholipase A(2) hydrolysis using specular neutron reflection and ellipsometry. Porcine pancreatic PLA(2) shows a long lag phase of several hours during which the enzyme binds to the bilayer surface, but only 5+/-3% of the lipids react before the onset of rapid hydrolysis. The… CONTINUE READING