Distribution of reaction products in phospholipase A2 hydrolysis.

  title={Distribution of reaction products in phospholipase A2 hydrolysis.},
  author={Hanna Pauliina Wacklin and Fredrik Tiberg and Giovanna Fragneto and Robert K Thomas},
  journal={Biochimica et biophysica acta},
  volume={1768 5},
We have monitored the composition of supported phospholipid bilayers during phospholipase A(2) hydrolysis using specular neutron reflection and ellipsometry. Porcine pancreatic PLA(2) shows a long lag phase of several hours during which the enzyme binds to the bilayer surface, but only 5+/-3% of the lipids react before the onset of rapid hydrolysis. The… CONTINUE READING