Distribution of prepubertal and adult goat oocyte cortical granules during meiotic maturation and fertilisation: ultrastructural and cytochemical study.

@article{Velilla2004DistributionOP,
  title={Distribution of prepubertal and adult goat oocyte cortical granules during meiotic maturation and fertilisation: ultrastructural and cytochemical study.},
  author={Esther Velilla and Dolors Izquierdo and Elisabet Rodr{\'i}guez-Gonz{\'a}lez and Manel Lopez-b{\'e}jar and Francesca Vidal and M T Paramio},
  journal={Molecular reproduction and development},
  year={2004},
  volume={68 4},
  pages={507-14}
}
The aim of this study was evaluate cortical granule (CG) distribution during in vitro maturation (IVM) and fertilisation of prepubertal goat oocytes compared to CG distribution of ovulated and in vitro fertilised oocytes from adult goats. Oocytes from prepubertal goats were recovered from a slaughterhouse and were matured in M199 with hormones and serum for 27 hr. Ovulated oocytes were collected from gonadotrophin treated Murciana goats. Frozen-thawed spermatozoa were selected by centrifugation… CONTINUE READING