Distribution of exogenous 25-hydroxycholesterol in Hep G2 cells between two different pools.

@article{Kisseleva1999DistributionOE,
  title={Distribution of exogenous 25-hydroxycholesterol in Hep G2 cells between two different pools.},
  author={A F Kisseleva and Ludmila E Goryunova and N. V. Medvedeva and Christian Alquier and Andrey D Morozkin and Misharin AYu},
  journal={FEBS letters},
  year={1999},
  volume={446 1},
  pages={163-8}
}
Binding of [26,27-(3)H]25-hydroxycholesterol (25HC) to human hepatoma Hep G2 cells was saturated within 120 min. Two intracellular pools of 25HC were identified in a pulse-chase experiment: (i) an exchangeable pool which was in dynamic equilibrium with 25HC in the medium (t(1/2) of reversible exchange 15 min) and (ii) an unexchangeable pool which remained… CONTINUE READING