Distribution of endocytosed molecules to intracellular acidic environments correlates with immunotoxin activity.

@article{Chignola1990DistributionOE,
  title={Distribution of endocytosed molecules to intracellular acidic environments correlates with immunotoxin activity.},
  author={Roberto Chignola and Marco Colombatti and L Dell'Arciprete and Carlo Candiani and Giuseppe Tridente},
  journal={International journal of cancer},
  year={1990},
  volume={46 6},
  pages={1117-23}
}
We have investigated the internalization to low pH intracellular compartments of transferrin (Tfn), diphtheria toxin (DT) and of anti-cell surface antibodies (MAb) by a cytofluorometric assay based on low pH quenching of fluorescein (FITC) emission. FITC-labelled Tfn, anti-CD3, anti-CD5 and anti-Thy 1.2 MAb internalization resulted in a progressively lower FITC quenching effect. Following internalization, a distinction could be made between molecules that enter low pH compartments without… CONTINUE READING

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