Distribution of annionic sites in glomerular basement membranes: their possible role in filtration and attachment.

@article{Caulfield1976DistributionOA,
  title={Distribution of annionic sites in glomerular basement membranes: their possible role in filtration and attachment.},
  author={John P. Caulfield and Marilyn Gist Farquhar},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1976},
  volume={73 5},
  pages={1646-50}
}
Lysozyme (pI = II) has been used to identify anionic sites in the glomerular capillary wall. A solution of 1-3% lysozyme was perfused into the left kidney at varying rates. After perfusion, the kidney was fixed in situ and processed for electron microscopy. Lysozyme was seen as an electron-dense deposit which was not present when succinylated lysozyme (pI = 4.5) or myoglobin (pI = 6.9) was perfused instead of native lysozyme. First, the epithelial cell plasma membrane was outlined by a 300-400… CONTINUE READING