Distribution of Pz-peptidase in bovine epididymal and ejaculated semen.

@article{Lessley1983DistributionOP,
  title={Distribution of Pz-peptidase in bovine epididymal and ejaculated semen.},
  author={B A Lessley and Duane L. Garner},
  journal={Biology of reproduction},
  year={1983},
  volume={28 2},
  pages={447-59}
}
Bovine epididymal or ejaculated semen was fractionated by density gradient centrifugation in Percoll, and seminal components recovered from the gradients were subjected to additional separation and washing steps. This procedure resulted in isolation of four major seminal constituents: particle-free extracellular fluid, washed light particulates, washed cytoplasmic droplets, and washed spermatozoa. When assayed using the Pz-peptide substrate, all the isolated seminal fractions contained… CONTINUE READING