Characteristics of the Structural Organization of the Ventral Posteromedial and Posterolateral Nuclei and the Reticular Nucleus of the Thalamus in Rats (an immunohistochemical study)
The aim of the present work was to identify columns in neocortical field S1 on frontal brain sections from white mongrel rats (n = 10) using immunocytochemical methods and antibodies to neuron proteins (synaptophysin, neurofilaments) and glial cells (glial fibrillary acidic protein (GFAP), myelin basic protein). Analysis of the expression of the main neurospecific antigens showed that on thin sections (4 μm), columns could be identified on the basis of groupings of astrocytes and neuron processes, i.e., axons and dendrites. Analysis of GFAP expression also showed that cortical layer I generally contained large numbers of large astrocytes with branching processes, as well as numerous small processes with high expression intensity. The synaptophysin content was high in all layers of the cortex, though the most intense reaction was seen in the molecular layer, as for reactions for GFAP. Expression of myelin basic protein identified radially distributed myelinated neuron processes in the cortex.