Distribution of Melissococcus plutonius in Honeybee Colonies with and without Symptoms of European Foulbrood

@article{Forsgren2004DistributionOM,
  title={Distribution of Melissococcus plutonius in Honeybee Colonies with and without Symptoms of European Foulbrood},
  author={Eva Forsgren and Anna Cassel Lundhagen and Anton Imdorf and Ingemar Fries},
  journal={Microbial Ecology},
  year={2004},
  volume={50},
  pages={369-374}
}
A sensitive hemi-nested polymerase chain reaction (PCR) was used for detection of Melissococcus plutonius, the causative agent of European foulbrood (EFB). Sampling was made in Switzerland, where EFB is a widespread disease and incidences have increased in recent years. Larvae from brood samples with and without clinical signs of disease (n = 92) and honey (n = 92) from the same colonies were investigated. Individual larvae (n = 60) and pupae (n = 30) from diseased brood in single colonies were… CONTINUE READING