Distribution of 1000 sequenced T-DNA tags in the Arabidopsis genome.

@article{Szabados2002DistributionO1,
  title={Distribution of 1000 sequenced T-DNA tags in the Arabidopsis genome.},
  author={L{\'a}szl{\'o} Szabados and Izabella Kov{\'a}cs and Attila Oberschall and Edit {\'A}brah{\'a}m and Ir{\'e}n Kerekes and Laura M. Zsigmond and R{\'e}ka Nagy and Martha A Alvarado and Inga Krasovskaja and M{\'o}nika G{\'a}l and Anik{\'o} Berente and George P. R{\'e}dei and Amit Ben Haim and Csaba Koncz},
  journal={The Plant journal : for cell and molecular biology},
  year={2002},
  volume={32 2},
  pages={233-42}
}
Induction of knockout mutations by T-DNA insertion mutagenesis is widely used in studies of plant gene functions. To assess the efficiency of this genetic approach, we have sequenced PCR amplified junctions of 1000 T-DNA insertions and analysed their distribution in the Arabidopsis genome. Map positions of 973 tags could be determined unequivocally, indicating that the majority of T-DNA insertions landed in chromosomal domains of high gene density. Only 4.7% of insertions were found in… CONTINUE READING
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