Distribution of 1000 sequenced T-DNA tags in the Arabidopsis genome.

  title={Distribution of 1000 sequenced T-DNA tags in the Arabidopsis genome.},
  author={L{\'a}szl{\'o} Szabados and Izabella Kov{\'a}cs and Attila Oberschall and Edit {\'A}brah{\'a}m and Ir{\'e}n Kerekes and Laura M. Zsigmond and R{\'e}ka Nagy and Martha A Alvarado and Inga Krasovskaja and M{\'o}nika G{\'a}l and Anik{\'o} Berente and George P. R{\'e}dei and Amit Ben Haim and Csaba Koncz},
  journal={The Plant journal : for cell and molecular biology},
  volume={32 2},
Induction of knockout mutations by T-DNA insertion mutagenesis is widely used in studies of plant gene functions. To assess the efficiency of this genetic approach, we have sequenced PCR amplified junctions of 1000 T-DNA insertions and analysed their distribution in the Arabidopsis genome. Map positions of 973 tags could be determined unequivocally, indicating that the majority of T-DNA insertions landed in chromosomal domains of high gene density. Only 4.7% of insertions were found in… CONTINUE READING
Highly Influential
This paper has highly influenced 12 other papers. REVIEW HIGHLY INFLUENTIAL CITATIONS

From This Paper

Figures, tables, and topics from this paper.


Publications citing this paper.
Showing 1-10 of 72 extracted citations

Flower Development

Methods in Molecular Biology • 2014
View 20 Excerpts
Highly Influenced

Mapped Ds/T-DNA launch pads for functional genomics in barley.

The Plant journal : for cell and molecular biology • 2006
View 7 Excerpts
Highly Influenced


Publications referenced by this paper.
Showing 1-10 of 46 references

In planta Agrobacterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants

N. Bechtold, J. Ellis, G. Pelletier
C.R. Acad. Sci. Paris, • 1993
View 7 Excerpts
Highly Influenced

MIPS: a database for genomes and protein sequences

Nucleic Acids Research • 2000
View 4 Excerpts
Highly Influenced

Identification of T-DNA insertions in Arabidopsis genes

L. Szabados, C. Koncz
In Genomics of Plants and Fungi (Prade, • 2002
View 5 Excerpts
Highly Influenced

Rapid identification of Arabidopsis insertion mutants by nonradioactive detection of T-DNA tagged genes

G. Rı́os, A. Lossow, B Hertel
Plant J • 2002
View 3 Excerpts
Highly Influenced

Arabidopsis gene knockout: phenotypes wanted.

Current opinion in plant biology • 2001
View 4 Excerpts
Highly Influenced

Similar Papers

Loading similar papers…